Arginine Vasopressin(4-8) Mobilizes Intracellular Calcium in C6 Glioma Cells

DONG Ming, XU Kan-Yan, ZHEN Xiao-Guang, DU Yu-Cang^*
( Shanghai Institute of Biochemistry, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China )

Abstract    To understand the mechanism of neurotrophic action of neuropeptide ZNC(C)PR, which could affect growth of C6 cells, fluorescent dye Fluo-3 and confocal laser scanning microscope were used to assay the intracellular calcium in C6 glioma cells. It was found that ZNC(C)PR and it's analogue NLPR could mobilize intracellular calcium in a dose-dependent manner. The ZNC(C)PR antagnist, ZDC(C)PR, could inhibit the process, and the extracellular calcium did not influence it.
Key words    ZNC(C)PR；calcium；C6 glioma cells；fluo3；confocal laser scanning microscope

^*Corresponding author：Tel,86-21-64374430-277；Fax,86-21-64338357；e-mail,[email protected]